File(s) not publicly available
Morphological observation of nuclear sub-localization of human angiogenin in HUVECs
journal contribution
posted on 2023-06-07, 22:36 authored by Jie Wu, Hong-Bin Zhang, Jie Wang, Tai Yang, Jiang Xian, Chuan-Hong Yang, Huang-Wen LaiAngiogenin, a potent angiogenic factor, was cloned and expressed by Escherichia coli and then purified with gel filtration chromatography. Approximately 90% pure angiogenin was obtained to generate a monoclonal antibody. Using western immunoblotting and ELISA, we confirmed that monoclonal antibody C46 secreted from hybridoma cells stably and specifically binds to angiogenin. The fused protein angiogenin-EGF was then expressed in HUVECs, and the subcellular localization of the recombinant protein was determined by confocal microscopy and TEM assay. Recombinant angiogenin was found to mainly concentrate in the pars granulosa of the nucleus, where the protein accumulates to form ribonucleoprotein particles. © 2008 Springer Science+Business Media B.V.
History
Publication status
- Published
Journal
Biotechnology LettersISSN
0141-5492External DOI
Issue
6Volume
30Page range
989-993Pages
5.0Department affiliated with
- Engineering and Design Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC