Newell, Katy V, Thomas, Derek P, Brekasis, Dimitris and Paget, Mark S B (2006) The RNA polymerase-binding protein RbpA confers basal levels of rifampicin resistance on Streptomyces coelicolor. Molecular Microbiology, 60 (3). pp. 687-696. ISSN 0950-382X

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Abstract

RbpA is an RNA polymerase-binding protein that occurs in the actinomycete family of bacteria and is regulated by the disulphide stress-response sigma factor, sigma(R), in Streptomyces coelicolor. Here we demonstrate that rbpA null mutants exhibit a slow-growth phenotype and are particularly sensitive to the transcription inhibitor rifampicin. Strikingly, transcription mapping experiments revealed that rbpA expression is induced upon exposure of S. coelicolor to rifampicin and that this, in part, involves an increase in the activity of sigma(R). In contrast, the ribosomal RNA operon promoter rrnDp3, which is recognized by the vegetative sigma factor sigma(HrdB), was strongly inhibited by rifampicin. Reconstitution of RNAP from an rbpA null mutant with purified RbpA revealed that RbpA stimulates transcription from rrnDp3, even in the presence of rifampicin. The data presented suggest that RbpA confers basal levels of rifampicin resistance and is a novel regulator of rRNA synthesis in S. coelicolor.

Item Type:	Article
Additional Information:	Newell was a PhD student, Thomas and Brekasis were post-doctoral fellows in Pagets lab when the work was performed. Paget conceived the project, Newell collected most data, and Paget wrote up the manuscript. It is the first description of a novel family of RNA polymerase binding proteins.
Schools and Departments:	School of Life Sciences > Biochemistry
Depositing User:	Mark Paget
Date Deposited:	06 Feb 2012 19:20
Last Modified:	20 Mar 2012 16:04
URI:	http://sro.sussex.ac.uk/id/eprint/20121

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