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Interaction of purified plant alternative oxidase from Arum maculatum with pyruvate

journal contribution
posted on 2023-06-07, 22:35 authored by Anthony Moore, C Affourtit, J Carré
Plant alternative oxidase (AOX) activity in isolated mitochondria is regulated by carboxylic acids, but reaction and regulatory mechanisms remain unclear. We show that activity of AOX protein purified from thermogenic Arum maculatum spadices is sensitive to pyruvate and glyoxylate but not succinate. Rapid, irreversible AOX inactivation occurs in the absence of pyruvate, whether or not duroquinol oxidation has been initiated, and is insensitive to duroquinone. Our data indicate that pyruvate stabilises an active conformation of AOX, increasing the population of active protein in a manner independent of reducing substrate and product, and are thus consistent with an exclusive effect of pyruvate on the enzymes apparent Vmax.

History

Publication status

  • Published

Journal

FEBS Letters

ISSN

0014-5793

Publisher

Elsevier

Issue

2

Volume

585

Page range

397-401

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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