NHEJ protects mycobacteria in stationary phase against the harmful effects of desiccation

Pitcher, Robert S, Green, Andrew J, Brzostek, Anna, Korycka-Machala, Malgorzata, Dziadek, Jaroslaw and Doherty, Aidan J (2007) NHEJ protects mycobacteria in stationary phase against the harmful effects of desiccation. DNA Repair, 6 (9). pp. 1271-1276. ISSN 1568-7864

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The physiological role of the non-homologous end-joining (NHEJ) pathway in the repair of DNA double-strand breaks (DSBs) was examined in Mycobacterium smegmatis using DNA repair mutants (¿recA, ¿ku, ¿ligD, ¿ku/ligD, ¿recA/ku/ligD). Wild-type and mutant strains were exposed to a range of doses of ionizing radiation at specific points in their life-cycle. NHEJ-mutant strains (¿ku, ¿ligD, ¿ku/ligD) were significantly more sensitive to ionizing radiation (IR) during stationary phase than wild-type M. smegmatis. However, there was little difference in IR sensitivity between NHEJ-mutant and wild-type strains in logarithmic phase. Similarly, NHEJ-mutant strains were more sensitive to prolonged desiccation than wild-type M. smegmatis. A ¿recA mutant strain was more sensitive to desiccation and IR during both stationary and especially in logarithmic phase, compared to wild-type strain, but it was significantly less sensitive to IR than the ¿recA/ku/ligD triple mutant during stationary phase. These data suggest that NHEJ and homologous recombination are the preferred DSB repair pathways employed by M. smegmatis during stationary and logarithmic phases, respectively.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Andrew Green
Date Deposited: 06 Feb 2012 19:17
Last Modified: 30 Mar 2012 08:36
URI: http://sro.sussex.ac.uk/id/eprint/19914
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