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Blodig, Wolfgang, Smith, Andrew T, Doyle, Wendy A and Piontek, Klaus (2001) Crystal structures of pristine and oxidatively processed lignin peroxidase expressed in Escherichia coli and of the W171F variant that eliminates the redox active tryptophan 171. Implications for the reaction mechanism. Journal of Molecular Biology, 305 (4). pp. 851-861. ISSN 0022-2836
Full text not available from this repository.Abstract
The heme enzyme lignin peroxidase (LiP) from the white rot fungus Phanerochaete chrysosporium contains a solvent exposed redox active tryptophan residue (Trp171) that carries a unique hydroxy group stereospecifically attached to its C-beta atom. A Trp171Phe mutant has no activity at all towards the substrate veratryl alcohol. The mechanism of veratryl alcohol oxidation involving beta -hydroxy-Trp171 is largely unknown. Here, we present the first crystal structures of LiP isozyme H8 at high resolution in its pristine non-hydroxylated form, of the C-beta-hydroxylated form, and of the Trp171Phe mutant using recombinantly expressed and refolded protein produced from Escherichia coli. As a consequence, all structures are unglycosylated. Structural changes in response to the mutation are marginal and allow us to attribute the complete lack of activity exclusively to the absence of the redox active indole side-chain. The origin of the stereospecificity of the Trp171 hydroxylation can be explained on structural grounds. A reaction mechanism involving Trp171 is proposed and the possible function of the modification is discussed. Another important result regarding the ongoing debate on the co-ordination state of the heme iron in the resting state is that the iron is six coordinate in all cases the data being collected at room temperature. The mean distance from the iron to the distal water Ligand is 2.18(+/-0.08) Angstrom. The radical scavenger orcinol was found to decrease radiation damage to the crystals, during data collection at room temperature.
| Item Type: | Article |
|---|---|
| Additional Information: | Recombinant lignin peroxidase produced at Sussex in ATS lab, mechanism proposed and paper co authored by ATS in collaboration with ETH group. First report of x-ray structures of recombinant lignin peroxidase with and without novel autocatalytic hydroxylation of the Cbeta of Trp 171, cited 23 times. |
| Schools and Departments: | School of Life Sciences > Biochemistry |
| Depositing User: | Andrew Trevor Smith |
| Date Deposited: | 06 Feb 2012 18:50 |
| Last Modified: | 20 Mar 2012 11:54 |
| URI: | http://sro.sussex.ac.uk/id/eprint/18555 |