University of Sussex
Browse
Kegel_etal_MS.pdf (389.39 kB)

X-irradiation of cells on glass slides has a dose doubling impact

Download (389.39 kB)
journal contribution
posted on 2023-06-07, 14:44 authored by Peter Kegel, Enriqueta Riballo, Martin Kühne, Penny Jeggo, Markus Löbrich
Immunofluorescence detection of ?H2AX foci is a widely used tool to quantify the induction and repair of DNA double-strand breaks (DSBs) induced by ionising radiation. We observed that X-irradiation of mammalian cells exposed on glass slides induced twofold higher foci numbers compared to irradiation with ?-rays. Here, we show that the excess ?H2AX foci after X-irradiation are produced from secondary radiation particles generated from the irradiation of glass slides. Both 120 kV X-rays and 137Cs ?-rays induce ~20 ?H2AX foci per Gy in cells growing on thin (~2 µm) plastic foils immersed in water. The same yield is obtained following ?-irradiation of cells growing on glass slides. However, 120 kV X-rays produce ~40 ?H2AX foci per Gy in cells growing on glass, twofold greater than obtained using cells irradiated on plastic surfaces. The same increase in ?H2AX foci number is obtained if the plastic foil on which the cells are grown is irradiated on a glass slide. Thus, the physical proximity to the glass material and not morphological differences of cells growing on different surfaces accounts for the excess ?H2AX foci. The increase in foci number depends on the energy and is considerably smaller for 25 kV relative to 120 kV X-rays, a finding which can be explained by known physical properties of radiation. The kinetics for the loss of foci, which is taken to represent the rate of DSB repair, as well as the Artemis dependent repair fraction, was similar following X- or ?-irradiation, demonstrating that DSBs induced by this range of treatments are repaired in an identical manner.

History

Publication status

  • Published

File Version

  • Accepted version

Journal

DNA Repair

ISSN

1568-7864

Publisher

Elsevier

Issue

11

Volume

6

Page range

1692-1697

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2008-09-30

First Open Access (FOA) Date

2018-05-08

First Compliant Deposit (FCD) Date

2019-07-02

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC