Kang, Ju-Gyeong, Paget, Mark S B, Seok, Yeong-Jae, Hahn, Mi-Young, Bae, Jae-Bum, Hahn, Ji-Sook, Kleanthous, Colin, Buttner, Mark J and Roe, Jung-Hye (1999) RsrA an anti-sigma factor regulated by redox change. EMBO Journal, 18 (5). pp. 4292-4298. ISSN 02614189

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Abstract

SigR (s(R)) is a sigma factor responsible for inducing the thioredoxin system in response to oxidative stress in the antibiotic-producing, Gram-positive bacterium Streptomyces coelicolor A3(2). Here we identify a redox-sensitive, s(R)-specific anti-sigma factor, RsrA, which binds s(R) and inhibits s(R)-directed transcription in vitro only under reducing conditions. Exposure to H2O2 or to the thiol-specific oxidant diamide caused the dissociation of the s(R)-RsrA complex, thereby allowing s(R)-dependent transcription. This correlated with intramolecular disulfide bond formation in RsrA. Thioredoxin was able to reduce oxidized RsrA, suggesting that s(R), RsrA and the thioredoxin system comprise a novel feedback homeostasis loop that senses and responds to changes in the intracellular thiol-disulfide redox balance.

Item Type:	Article
Additional Information:	This was the first description of a new family of anti-sigma factors and RsrA was the first-described anti-sigma factor controlled by redox change.
Schools and Departments:	School of Life Sciences > Biochemistry
Depositing User:	Mark Paget
Date Deposited:	06 Feb 2012 18:42
Last Modified:	14 Jun 2012 09:03
URI:	http://sro.sussex.ac.uk/id/eprint/17889

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