The trans Golgi network is lost from cells infected with African Swine Fever

McCrossan, Mari, Windsor, Miriam, Ponnambalam, Sreenivasan, Armstrong, John and Wileman, Thomas (2001) The trans Golgi network is lost from cells infected with African Swine Fever. Journal of Virology, 75 (23). pp. 11755-11765. ISSN 0022-538X

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The cellular secretory pathway is important during the assembly and envelopment of viruses and also controls the transport of host proteins, such as cytokines and major histocompatibility proteins, that function during the elimination of viruses by the immune system. African swine fever virus (ASFV) encodes at least 26 proteins with stretches of hydrophobic amino acids suggesting entry into the secretory pathway (R. J. Yanez, J. M. Rodriguez, M. L. Nogal, L. Yuste, C. Enriquez, J. F. Rodriguez, and E. Vinuela, Virology 208:249-278, 1995). To predict how and where these potential membrane proteins function, we have studied the integrity of the secretory pathway in cells infected with ASFV. Remarkably, ASFV caused complete loss of immunofluorescence signal for the trans Golgi network (TGN) marker protein TGN46 and dispersed the AP1 TGN adapter complex. Loss of TGN46 signal was not due to degradation of TGN46, suggesting redistribution of TGN46 to other membrane compartments. ASFV markedly slowed transport of cathepsin D to lysosomes, demonstrating that loss of TGN structure correlated with loss of TGN function. ASFV shows a tropism for macrophages, and it is possible that ASFV compromises TGN function to augment the activity of viral membrane proteins or to suppress the function of host immunoregulatory proteins.

Item Type: Article
Additional Information: Part of long-term collaboration on trafficking and viruses. Contributed significantly to microscopy and cell biological anlysis. First author a co-supervisee.
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: John Armstrong
Date Deposited: 06 Feb 2012 18:37
Last Modified: 20 Mar 2012 09:24
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