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The trans Golgi network is lost from cells infected with African Swine Fever
journal contribution
posted on 2023-06-07, 20:44 authored by Mari McCrossan, Miriam Windsor, Sreenivasan Ponnambalam, John Armstrong, Thomas WilemanThe cellular secretory pathway is important during the assembly and envelopment of viruses and also controls the transport of host proteins, such as cytokines and major histocompatibility proteins, that function during the elimination of viruses by the immune system. African swine fever virus (ASFV) encodes at least 26 proteins with stretches of hydrophobic amino acids suggesting entry into the secretory pathway (R. J. Yanez, J. M. Rodriguez, M. L. Nogal, L. Yuste, C. Enriquez, J. F. Rodriguez, and E. Vinuela, Virology 208:249-278, 1995). To predict how and where these potential membrane proteins function, we have studied the integrity of the secretory pathway in cells infected with ASFV. Remarkably, ASFV caused complete loss of immunofluorescence signal for the trans Golgi network (TGN) marker protein TGN46 and dispersed the AP1 TGN adapter complex. Loss of TGN46 signal was not due to degradation of TGN46, suggesting redistribution of TGN46 to other membrane compartments. ASFV markedly slowed transport of cathepsin D to lysosomes, demonstrating that loss of TGN structure correlated with loss of TGN function. ASFV shows a tropism for macrophages, and it is possible that ASFV compromises TGN function to augment the activity of viral membrane proteins or to suppress the function of host immunoregulatory proteins.
History
Publication status
- Published
Journal
Journal of VirologyISSN
0022-538XIssue
23Volume
75Page range
11755-11765Pages
11.0Department affiliated with
- Biochemistry Publications
Notes
Part of long-term collaboration on trafficking and viruses. Contributed significantly to microscopy and cell biological anlysis. First author a co-supervisee.Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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