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Molecular characterization of major histocompatibility complex class II alleles in the common frog, Rana temporaria
journal contribution
posted on 2023-06-07, 19:18 authored by I Zeisset, T J C BeebeeMajor histocompatibility complex (MHC) class II genes, which play a major role in the immune system response, are some of the most polymorphic genes in vertebrates. We developed polymerase chain reaction primers for part of the second exon of an expressed MHC class II gene in the common frog, Rana temporaria. We genotyped this locus in five frog populations in southeast England and detected eight alleles in 215 individuals. Five or six alleles were detected in each population with a maximum of two alleles per individual, indicating that only a single locus was amplified. We also inferred the possible existence of a null allele. There were 23 variable nucleotide sites (out of 136) and 13 variable amino acid sites (out of 44), many of which corresponded to amino acids involved in antigen recognition. We detected a significant excess of nonsynonymous substitutions at antigen binding sites, indicating that this gene is under positive selection. The level of variation we found was similar to that in other amphibian MHC class II loci, such as those in Bombina bombina, Xenopus laevis and Ambystoma tigrinum.
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Publication status
- Published
Journal
Molecular Ecology ResourcesISSN
1755-098XExternal DOI
Issue
3Volume
9Page range
738-745Pages
8.0Department affiliated with
- Evolution, Behaviour and Environment Publications
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- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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