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Stn1-Ten1 and Taz1 independently promote replication of subtelomeric fragile sequences in fission yeast
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posted on 2023-06-10, 07:12 authored by Mélina Vaurs, Karel Naiman, Chaïnez Bouabboune, Sudhir Rai, Katarzyna Ptasinska, Marion Rives, Samah Matmati, Antony CarrAntony Carr, Vincent Géli, Stéphane CoulonEfficient replication of terminal DNA is crucial to maintain telomere stability. In fission yeast, Taz1 and the Stn1-Ten1 (ST) complex play prominent roles in DNA-ends replication. However, their function remains elusive. Here, we have analyzed genome-wide replication and show that ST does not affect genome-wide replication but is crucial for the efficient replication of a subtelomeric region called STE3-2. We further show that, when ST function is compromised, a homologous recombination (HR)-based fork restart mechanism becomes necessary for STE3-2 stability. While both Taz1 and Stn1 bind to STE3-2, we find that the STE3-2 replication function of ST is independent of Taz1 but relies on its association with the shelterin proteins Pot1-Tpz1-Poz1. Finally, we demonstrate that the firing of an origin normally inhibited by Rif1 can circumvent the replication defect of subtelomeres when ST function is compromised. Our results help illuminate why fission yeast telomeres are terminal fragile sites.
History
Publication status
- Published
File Version
- Published version
Journal
Cell ReportsISSN
2211-1247Publisher
ElsevierExternal DOI
Issue
6Volume
42Page range
a112537Event location
United StatesDepartment affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2023-05-31First Open Access (FOA) Date
2023-05-31First Compliant Deposit (FCD) Date
2023-05-31Usage metrics
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