molecules-28-03844.pdf (3.56 MB)
Studies of dopamine oxidation process by atmospheric pressure glow discharge mass spectrometry
journal contribution
posted on 2023-06-10, 06:57 authored by Dongli Dai, Yueqin Zhu, Zhenli Zhu, Rong Qian, Shangjun Zhuo, Anqi Liu, Xian Li, Wei Li, Qiao ChenQiao ChenAn atmospheric pressure glow discharge ionisation source was constructed and utilized to study the dopamine (DA) oxidation process coupling with mass spectrometry. During the DA oxidation process catalysed by polyphenol oxidase (PPO), six cationic intermediates were directly detected by the atmospheric pressure glow discharge mass spectrometry (APGD-MS). Combined with tandem mass spectrometry, the structures of the dopamine o-semiquinone radical (DASQ) and leukodopaminochrome radical (LDAC?) intermediates and structures of the isomers of dopaminochrome (DAC) and 5,6-dihydroxyindole (DHI) were further characterised with the introduction of 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO) and deuterium oxide (D2O) to APGD-MS. Meanwhile, UV–Vis studies confirmed the important role of PPO in catalyzing the DA oxidation reaction. Based on APGD-MS studies, a possible mechanism could be proposed for DA oxidation catalysed by PPO. Furthermore, APGD-MS could provide possibilities for the effective detection and characterisation of short-lived intermediates, even in complicated systems.
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- Published
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- Published version
Journal
MoleculesISSN
1420-3049Publisher
MDPI AGExternal DOI
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9Volume
28Page range
a3844 1-10Department affiliated with
- Chemistry Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2023-05-03First Open Access (FOA) Date
2023-05-03First Compliant Deposit (FCD) Date
2023-05-03Usage metrics
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