Zn doped paper.pdf (7.9 MB)
Zn-doped chitosan/alginate multilayer coatings on porous hydroxyapatite scaffold with osteogenic and antibacterial properties
journal contribution
posted on 2023-06-10, 06:08 authored by Zhijing He, Chen Jiao, Junnan Wu, Jiasen Gu, Huixin Liang, Lida Shen, Youwen Yang, Zongjun Tian, Chang WangChang Wang, Qing JiangPorous hydroxyapatite (HA) scaffolds prepared by three-dimensional (3D) printing have wide application prospects owing to personalized structural design and excellent biocompatibility. However, the lack of antimicrobial properties limits its widespread use. In this study, a porous ceramic scaffold was fabricated by digital light processing (DLP) method. The multilayer chitosan/alginate composite coatings prepared by layer-by-layer method were applied to scaffolds and Zn2+ was doped into coatings in the form of ion crosslinking. The chemical composition and morphology of coatings were characterized by scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). Energy dispersive spectroscopy (EDS) analysis demonstrated that Zn2+ was uniformly distributed in the coating. Besides, the compressive strength of coated scaffolds (11.52 ± 0.3 MPa) was slightly improved compared with that of bare scaffolds (10.42 ± 0.56 MPa). The result of soaking experiment indicated that coated scaffolds exhibited delayed degradation. In vitro experiments demonstrated that within the limits of concentration, a higher Zn content in the coating has a stronger capacity to promote cell adhesion, proliferation and differentiation. Although excessive release of Zn2+ led to cytotoxicity, it presented a stronger antibacterial effect against Escherichia coli (99.4%) and Staphylococcus aureus (93%).
History
Publication status
- Published
File Version
- Published version
Journal
International Journal of BioprintingISSN
2424-7723Publisher
Whioce Publishing Pte LtdExternal DOI
Issue
2Volume
9Page range
292-305Pages
14.0Department affiliated with
- Engineering and Design Publications
Notes
Porous hydroxyapatite scaffold, Multi-layer polymercoating, Zn doping, Osteogenic property, Antibacterial propertyFull text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2023-02-02First Open Access (FOA) Date
2023-02-02First Compliant Deposit (FCD) Date
2023-02-01Usage metrics
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