Al-Hassan, Leena, Yokoyama, Maho, Cantillon, Daire, Altayb, Hisham, Osman, Einas, Wille, Julia, Seifert, Harald and Higgins, Paul (2023) Klebsiella pneumonia in Sudan: multidrug resistance, polyclonal dissemination, and virulence. Antibiotics, 12 (2). pp. 1-13. ISSN 2079-6382

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Abstract

The emergence and global expansion of hyper-virulent and multidrug resistant (MDR) Klebsiella pneumoniae is an increasing healthcare threat worldwide. The epidemiology of MDR K. pneumoniae is under-characterized in many parts of the world, particularly Africa. In this study, K. pneumoniae isolates from hospitals in Khartoum, Sudan, have been whole-genome sequenced to investigate their molecular epidemiology, virulence, and resistome profiles. Eighty-six K. pneumoniae were recovered from patients in five hospitals in Khartoum between 2016 and 2020. Antimicrobial susceptibility was performed by disk-diffusion and broth microdilution. All isolates underwent whole genome sequencing using Illumina MiSeq; cgMLST was determined using Ridom SeqSphere+, and 7-loci MLST virulence genes and resistomes were identified. MDR was observed at 80%, with 35 isolates (41%) confirmed carbapenem-resistant. Thirty-seven sequence types were identified, and 14 transmission clusters (TC). Five of these TCs involved more than one hospital. Ybt9 was the most common virulence gene detected, in addition to some isolates harbouring iuc and rmp1. There is a diverse population of K. pneumoniae in Khartoum hospitals, harbouring multiple resistance genes, including genes coding for ESBLs, carbapenemases, and aminoglycoside-modifying enzymes, across multiple ST’s. The majority of isolates were singletons and transmissions were rare.

Item Type:	Article
Keywords:	Klebsiella pneumoniae, multi-drug resistance, transmission, Sudan
Schools and Departments:	Brighton and Sussex Medical School > Global Health and Infection
Research Centres and Groups:	Brighton and Sussex Centre for Global Health Research
SWORD Depositor:	Mx Elements Account
Depositing User:	Mx Elements Account
Date Deposited:	23 Jan 2023 11:00
Last Modified:	23 Jan 2023 11:15
URI:	http://sro.sussex.ac.uk/id/eprint/110281

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