f1148f36-b3ad-412a-b16f-2530a7f6388d_18327_-_alex_herbert_(1).pdf (698.31 kB)
GDSC SMLM: single-molecule localisation microscopy software for ImageJ
journal contribution
posted on 2023-06-10, 05:28 authored by Thomas Etheridge, Antony CarrAntony Carr, Alex D HerbertSingle-molecule localisation microscopy (SMLM) uses software to extract super-resolved positions from microscope images of fluorescent molecules. These localisations can then be used to render super-resolution images or analysed to extract information about molecular behaviour. The GDSC SMLM software provides a set of tools for analysing SMLM data in a single cross-platform environment. The software identifies fluorescent molecules in raw microscope images and localises their positions using stages of spot detection, spot fitting and spot rejection. The resulting localisation data set can then be visualised, cropped and filtered. A suite of downstream analysis tools enable the user to perform single-particle tracking, cluster analysis and drift correction. In addition, GDSC SMLM also provides utility tools that enable modelling of EM-CCD and sCMOS cameras as well as point spread functions (PSFs) for data simulation. The software is written in Java and runs as a collection of plugins for the ImageJ software.
History
Publication status
- Published
File Version
- Published version
Journal
Wellcome Open ResearchISSN
2398-502XPublisher
F1000 Research LtdExternal DOI
Volume
7Page range
241Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2022-11-15First Open Access (FOA) Date
2022-11-24First Compliant Deposit (FCD) Date
2022-11-24Usage metrics
Categories
No categories selectedLicence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC