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GDSC SMLM: single-molecule localisation microscopy software for ImageJ

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posted on 2023-06-10, 05:28 authored by Thomas Etheridge, Antony CarrAntony Carr, Alex D Herbert
Single-molecule localisation microscopy (SMLM) uses software to extract super-resolved positions from microscope images of fluorescent molecules. These localisations can then be used to render super-resolution images or analysed to extract information about molecular behaviour. The GDSC SMLM software provides a set of tools for analysing SMLM data in a single cross-platform environment. The software identifies fluorescent molecules in raw microscope images and localises their positions using stages of spot detection, spot fitting and spot rejection. The resulting localisation data set can then be visualised, cropped and filtered. A suite of downstream analysis tools enable the user to perform single-particle tracking, cluster analysis and drift correction. In addition, GDSC SMLM also provides utility tools that enable modelling of EM-CCD and sCMOS cameras as well as point spread functions (PSFs) for data simulation. The software is written in Java and runs as a collection of plugins for the ImageJ software.

History

Publication status

  • Published

File Version

  • Published version

Journal

Wellcome Open Research

ISSN

2398-502X

Publisher

F1000 Research Ltd

Volume

7

Page range

241

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2022-11-15

First Open Access (FOA) Date

2022-11-24

First Compliant Deposit (FCD) Date

2022-11-24

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