GDSC SMLM: single-molecule localisation microscopy software for ImageJ

Etheridge, Thomas J, Carr, Antony M and Herbert, Alex D (2022) GDSC SMLM: single-molecule localisation microscopy software for ImageJ. Wellcome Open Research, 7. p. 241. ISSN 2398-502X

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Abstract

Single-molecule localisation microscopy (SMLM) uses software to extract super-resolved positions from microscope images of fluorescent molecules. These localisations can then be used to render super-resolution images or analysed to extract information about molecular behaviour. The GDSC SMLM software provides a set of tools for analysing SMLM data in a single cross-platform environment. The software identifies fluorescent molecules in raw microscope images and localises their positions using stages of spot detection, spot fitting and spot rejection. The resulting localisation data set can then be visualised, cropped and filtered. A suite of downstream analysis tools enable the user to perform single-particle tracking, cluster analysis and drift correction. In addition, GDSC SMLM also provides utility tools that enable modelling of EM-CCD and sCMOS cameras as well as point spread functions (PSFs) for data simulation. The software is written in Java and runs as a collection of plugins for the ImageJ software.

Item Type: Article
Keywords: Bioengineering, Generic health relevance
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
SWORD Depositor: Mx Elements Account
Depositing User: Mx Elements Account
Date Deposited: 15 Nov 2022 09:17
Last Modified: 24 Nov 2022 15:30
URI: http://sro.sussex.ac.uk/id/eprint/109061

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