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Quantitative nuclear proteomics reveals new phenotypes altered in lymphoblastoid cells
journal contribution
posted on 2023-06-10, 04:55 authored by Paul Brennan, Angharad M Shore, Matthew Clement, Saman Hewamana, Catrin M Jones, Peter Giles, Christopher Fegan, Christopher PepperChristopher Pepper, Ian A BrewisB-lymphocytes are essential for the production of antibodies to fight pathogens and are the cells of origin in 95% of human lymphomas. During their activation, and immortalisation by Epstein - Barr virus (EBV) which contributes to human cancers, B-lymphocytes undergo dramatic changes in cell size and protein content. This study was initiated to compare the proteome of two B-cell lines, from the same individual, that reflect different patterns of activation, one is EBV negative and the other is EBV positive. Using isobaric tags, LC-MALDI TOF-TOF and subcellular fractionation, we quantified 499 proteins from B-cells. From a detergent lysed protein extract, we identified 34 proteins that were differentially expressed in EBV-immortalised B-cells. By analysing a nuclear extract, we identified a further 29 differentially expressed proteins with only four proteins shared between the two extracts, illustrating the benefit of subcellular fractionation. This analysis has identified proteins involved in the cytoskeletal phenotype of activated B-cells and the increased antigen recognition in EBV-immortalised cells. Importantly, we have also identified new regulators of transcription and changes in ribonuclear proteins that may contribute to the increased cell size and immortalisation of lymphoblastoid cells. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
History
Publication status
- Published
Journal
Proteomics - Clinical ApplicationsISSN
1862-8346Publisher
WileyExternal DOI
Issue
3Volume
3Page range
359-369Event location
GermanyDepartment affiliated with
- Clinical and Experimental Medicine Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2022-09-30Usage metrics
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