Carr, Antony, MacNeil, S. A., Hayles, J. and Nurse, P. (1989) Molecular cloning and sequence analysis of mutant alleles of the fission yeast cdc2 protein kinase gene: implications for cdc2+ protein structure and function. Molecular and General Genetics MGG, 218. pp. 41-49. ISSN 0026-8925

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Abstract

The cdc2+ gene function plays a central role in the control of the mitotic cell cycle of the fission yeast Schizosaccharomyces pombe. Recessive temperature-sensitive mutations in the cdc2 gene cause cell cycle arrest when shifted to the restrictive temperature, while a second class of mutations within the cdc2 gene causes a premature advancement into mitosis. Previously the cdc2+ gene has been cloned and has been shown to encode a 34 kDa phosphoprotein with in vitro protein kinase activity. Here we describe the cloning of 11 mutant alleles of the cdc2 gene using two simple methods, one of which is presented here for the first time. We have sequenced these alleles and find a variety of single amino acid substitutions mapping throughout the cdc2 protein. Analysis of these mutations has identified a number of regions within the cdc2 protein that are important for cdc2+ activity and regulation. These include regions which may be involved in the interaction of the cdc2+ gene product with the proteins encoded by the wee1+, cdc13+ and suc1+ genes.

Item Type:	Article
Subjects:	Q Science > QP Physiology
Depositing User:	Gee Wheatley
Date Deposited:	17 Apr 2007
Last Modified:	16 Sep 2019 10:07
URI:	http://sro.sussex.ac.uk/id/eprint/1081
Google Scholar:	80 Citations

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