excli2022-5266.pdf (1.19 MB)
Correlation of MIR-24-3P and MIR-595 expression with CCL3, CCL4, IL-1?, TNF?IP3, and NF-?BI? genes in PBMCS of patients with coronary artery disease
journal contribution
posted on 2023-06-10, 04:40 authored by Shakiba Kazemian, Reza Ahmadi, Gordon FernsGordon FernsInflammation has been well recognized to play an important role in developing coronary artery disease (CAD). By regulating essential genes in this pathway post-transcriptionally, MicroRNAs (miRNAs) may help or hinder the development of atherosclerotic lesions. The aim of this study was to investigate the expression of miR-24-3p, miR-595, CCL3, CCL4, IL-1ß, TNFaIP3, and NF-?BIa in the peripheral blood mononuclear cells (PBMCs) of CAD and control groups and to examine whether any correlation exists between the expression of miRs and genes in CAD group. A total of 168 subjects (84 CAD subjects and 84 control subjects) were examined in this research. Expression levels of miR-24-3p, miR-595, CCL3, CCL4, IL-1ß, TNFaIP3, and NF-?BIa in PBMCs were measured using the real-time PCR technique. A comparison of the CAD group with the control group indicated significantly increased expression levels of CCL3, CCL4, and IL-1ß (Fold Change (FC) =4, P=0.009; FC=2.9, P=0.01; FC=1.8, P=0.019, respectively) and remarkably reduced expression levels of TNFaIP3 and NF?BIa (FC=-1.4, P=0.03 and FC=-5.9, P=0.001, respectively). Moreover, the expression levels of miR-24-3p downregulated (FC=-2.5, P=0.005) and miR-595 upregulated (FC=1.9, P=0.009) in the CAD group. There was a statistical correlation between the number of clogged arteries with expression levels of miR-24-3p, miR-595, CCL3, CCL4, IL-1ß, TNFaIP3, and NF-?BIa in the CAD group. Also, there was a statistical correlation between expression levels of miR-24-3p and miR-595 with CCL3, CCL4, IL-1ß, TNFaIP3, and NF-?BIa gene expression in the CAD group. In CAD patients, decreased expression of miR-24-3p and increased expression of miR-595 may aid the progression of atherosclerotic plaques by regulating CCL3, CCL4, IL-1ß, TNFaIP3, and NF?BIa gene expression.
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Publication status
- Published
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- Published version
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EXCLI JournalISSN
1611-2156Publisher
IfADo - Leibniz Research Centre for Working Environment and Human Factors, DortmundExternal DOI
Volume
21Page range
1-12Department affiliated with
- Division of Medical Education Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2022-09-07First Open Access (FOA) Date
2022-10-05First Compliant Deposit (FCD) Date
2022-09-07Usage metrics
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