Construction, purification and immumogenicity of antigen-antibody-LTB complexes

Green, E A, Botting, C, Webb, H M, Hirst, T R and Randall, R E (1996) Construction, purification and immumogenicity of antigen-antibody-LTB complexes. Vaccine, 14. pp. 949-958. ISSN 0264-410X

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An oligonucleotide, encoding a short epitope peptide tag, termed Pk, was inserted at the 3'-end of the gene coding B-subunit of Escherichia coli heat-labile enterotoxin (LTB). The presence of the Pk epitope on LTB-PK was used to construct novel macromolecular assemblies comprising LTB-Pk, an anti-Pk mAb, (mAb SV5-P-k) and Pk-linked recombinant SIV proteins. The 1:1:1 stoichiometry of such complexes was ensured by binding LTB-Pk to one ann of mAb SVS-P-k and an SIV-Pk antigen to the other arm of the antibody. Such SIV-mAb-LTB macromolecular complexes bound to GM1-ganglioside in vitro, and when immunized systemically into mice were highly immunogenic, inducing both humoral and cell-mediated responses to the recombinant SIV antigens.

Item Type: Article
Keywords: Amino Acid Sequence;Animals;Antibodies, Monoclonal;Antibody Formation;Antigen-Antibody Reactions;Bacterial Toxins;Base Sequence;Binding Sites, Antibody;Enterotoxins;Escherichia coli Proteins;G(M1) Ganglioside;Immunity, Cellular;Mice;Mice, Inbred BALB C;Molecular Sequence Data;Simian Immunodeficiency Virus;Vaccines, Synthetic
Schools and Departments: School of Life Sciences > Biochemistry
SWORD Depositor: Mx Elements Account
Depositing User: Mx Elements Account
Date Deposited: 01 Jul 2022 08:16
Last Modified: 01 Jul 2022 08:16
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