Takamata, Akira, Nishimura, Yuri, Oka, Ayano, Nagata, Mayuna, Kosugi, Natsumi, Eguchi, Sayaka, Negishi, Hiroko and Morimoto, Keiko (2022) Endogenous androgens diminish food intake and activation of orexin A neurons in response to reduced glucose availability in male rats. Nutrients, 14 (6). a1235 1-11. ISSN 2072-6643

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Abstract

Sex steroids modify feeding behavior and body weight regulation, and androgen reportedly augments food intake and body weight gain. To elucidate the role of endogenous androgens in the feeding regulation induced by reduced glucose availability, we examined the effect of gonadectomy (orchiectomy) on food intake and orexin A neuron’s activity in the lateral hypothalamic/perifornical area (LH/PFA) in response to reduced glucose availability (glucoprivation) induced by 2-deoxy-d-glucose (2DG) administration in male rats. Rats (7W) were bilaterally orchiectomized (ORX group) or sham operated (Sham group). Seventeen days after the surgery, food intake response to 2DG (400 mg/kg, i.v.) was measured for 4 h after the infusion. The same experiment was performed for the immunohistochemical examination of c-Fos-expressing orexin A neurons in the LH/PFA and c-Fos expression in the arcuate nucleus (Arc). Food intake induced by glucoprivation was greater in the ORX group than the Sham group, and the glucoprivation-induced food intake was inversely correlated with plasma testosterone concentration. Glucoprivation stimulated c-Fos expression of the orexin A neurons at the LH/PFA and c-Fos expression in the dorsomedial Arc. The number and percentage of c-Fos-expressing orexin A neurons in the LH/PFA and c-Fos expression in the dorsomedial Arc were significantly higher in the ORX group than the Sham group. This indicates that endogenous androgen, possibly testosterone, diminishes the food intake induced by reduced glucose availability, possibly via the attenuated activity of orexin A neuron in the LH/PFA and neurons in the dorsomedial Arc.

Item Type:	Article
Keywords:	2-deoxy-d-glucose, arcuate nucleus, feeding behavior, glucoprivation, glucose availability, lateral hypothalamic area, orchiectomy, orexin, perifornical area, testosterone, Androgens, Animals, Eating, Glucose, Intracellular Signaling Peptides and Proteins, Male, Neurons, Neuropeptides, Orexins, Rats
Schools and Departments:	School of Life Sciences > Neuroscience
SWORD Depositor:	Mx Elements Account
Depositing User:	Mx Elements Account
Date Deposited:	26 May 2022 09:17
Last Modified:	26 May 2022 09:30
URI:	http://sro.sussex.ac.uk/id/eprint/106105

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