Wooller, Sarah, Anagnostopoulou, Aikaterini, Kuropka, Benno, Crossley, Michael, Benjamin, Paul R, Pearl, Frances, Kemenes, Ildiko, Kemenes, György and Eravci, Murat (2022) A combined bioinformatics and LC-MS-based approach for the development and benchmarking of a comprehensive database of Lymnaea CNS proteins. Journal of Experimental Biology, 225 (7). pp. 1-7. ISSN 0022-0949

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Abstract

Applications of key technologies in biomedical research, such as qRT-PCR or LC-MS-based proteomics, are generating large biological (-omics) datasets which are useful for the identification and quantification of biomarkers in any research area of interest. Genome, transcriptome and proteome databases are already available for a number of model organisms including vertebrates and invertebrates. However, there is insufficient information available for protein sequences of certain invertebrates, such as the great pond snail Lymnaea stagnalis, a model organism that has been used highly successfully in elucidating evolutionarily conserved mechanisms of memory function and dysfunction. Here, we used a bioinformatics approach to designing and benchmarking a comprehensive central nervous system (CNS) proteomics database (LymCNS-PDB) for the identification of proteins from the CNS of Lymnaea by LC-MS-based proteomics. LymCNS-PDB was created by using the Trinity TransDecoder bioinformatics tool to translate amino acid sequences from mRNA transcript assemblies obtained from a published Lymnaea transcriptomics database. The blast-style MMSeq2 software was used to match all translated sequences to UniProtKB sequences for molluscan proteins, including those from Lymnaea and other molluscs. LymCNS-PDB contains 9628 identified matched proteins that were benchmarked by performing LC-MS-based proteomics analysis with proteins isolated from the Lymnaea CNS. MS/MS analysis using the LymCNS-PDB database led to the identification of 3810 proteins. Only 982 proteins were identified by using a non-specific molluscan database. LymCNS-PDB provides a valuable tool that will enable us to perform quantitative proteomics analysis of protein interactomes involved in several CNS functions in Lymnaea, including learning and memory and age-related memory decline.

Item Type:	Article
Keywords:	Lymnaea, Bioinformatics, Central nervous system, Liquid chromatography–mass spectrometry, Proteomics database, Animals, Benchmarking, Central Nervous System, Chromatography, Liquid, Computational Biology, Proteins, Tandem Mass Spectrometry
Schools and Departments:	School of Life Sciences > Biochemistry School of Life Sciences > Neuroscience
SWORD Depositor:	Mx Elements Account
Depositing User:	Mx Elements Account
Date Deposited:	06 May 2022 10:18
Last Modified:	06 May 2022 10:30
URI:	http://sro.sussex.ac.uk/id/eprint/105760

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