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Association of ANGPTL3 polymorphisms with high-density lipoprotein cholesterol uptake capacity in patients with cardiovascular disease
journal contribution
posted on 2023-06-10, 03:17 authored by Malihe Aghasizadeh, Mina Nosrati, Maryam Saberi-Karimian, Hamideh Safarian, Parisa Assadian, Ensieh Akbarpour, Amirhosein Sahebkar, Amir Avan, Gordon FernsGordon Ferns, Tooba Kazemi, Ebrahim Miri-Moghaddam, Majid Ghayour-MobarhanIntroduction Previous studies have shown the importance of angiopoietin-like 3 (ANGPTL3) as a modulator of lipid profiles. Cholesterol uptake capacity (CUC) is one means for assessing high-density lipoprotein (HDL) functionality. This study for the first time has investigated the relationship between genetic ANGPTL3 polymorphism and CUC in patients with cardiovascular disease. Methods Five hundred three subjects comprising 350 healthy subjects and 153 individuals who developed a cardiovascular disease (CVD) event during follow-up were recruited as part of the Mashhad Stroke and Heart Atherosclerotic Disorder (MASHAD) cohort study. A modified CUC method was used to determine the CUC of serum samples. Applied amplification refractory mutation system PCR was performed for ANGPTL3 variants genotyping including: rs10789117, rs1748195, and rs11207997. Sanger sequencing was applied to confirm the genotypes. Results The results showed that there was a significant relationship between the rs1748195 genotypes and HDL concentration in the CVD group (p = 0.02). Moreover, individuals with a GG genotype of the rs1748195 were associated with a lower risk of CVD (OR = 0.49, 95% CI = 0.24–0.98, p = 0.04) compared with CC genotype in the CUC = 1.7 a.u subgroup. Moreover, the CT genotype of rs11207997 was associated with a lower risk of CVD (OR = 0.74, 95% CI = 0.41–1.3, p = 0.01) compared with CC genotype in CUC > 1.7 a.u subgroup. Conclusion The results showed that the CT genotype of the rs11207997 variant was associated with a lower risk of incident CVD in patients with higher HDL functionality. As well, the rs1748195 gene variant may contribute to a reduced risk of CVD.
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Publication status
- Published
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- Published version
Journal
Journal of Clinical Laboratory AnalysisISSN
0887-8013Publisher
WileyExternal DOI
Issue
12Volume
35Page range
1-8Article number
a23980Event location
United StatesDepartment affiliated with
- Division of Medical Education Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2022-04-29First Open Access (FOA) Date
2022-04-29First Compliant Deposit (FCD) Date
2022-04-29Usage metrics
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