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2022 - Janiak et al - Nature Comms.pdf (25.53 MB)

Non-telecentric two-photon microscopy for 3D random access mesoscale imaging

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posted on 2023-06-10, 02:30 authored by Filip JaniakFilip Janiak, Philipp Bartel, Michael Bale, Takeshi YoshimatsuTakeshi Yoshimatsu, Emilia KomulainenEmilia Komulainen, Mingyi Zhou, Kevin StarasKevin Staras, L L Prieto-Godino, T Euler, Miguel Maravall RodriguezMiguel Maravall Rodriguez, Thomas BadenThomas Baden
Diffraction-limited two-photon microscopy permits minimally invasive optical monitoring of neuronal activity. However, most conventional two-photon microscopes impose significant constraints on the size of the imaging field-of-view and the specific shape of the effective excitation volume, thus limiting the scope of biological questions that can be addressed and the information obtainable. Here, employing a non-telecentric optical design, we present a low-cost, easily implemented and flexible solution to address these limitations, offering a several-fold expanded three-dimensional field of view. Moreover, rapid laser-focus control via an electrically tunable lens allows near-simultaneous imaging of remote regions separated in three dimensions and permits the bending of imaging planes to follow natural curvatures in biological structures. Crucially, our core design is readily implemented (and reversed) within a matter of hours, making it highly suitable as a base platform for further development. We demonstrate the application of our system for imaging neuronal activity in a variety of examples in zebrafish, mice and fruit flies.

Funding

Anisotropic retinal circuits for processing of colour and space in nature; G2397; BBSRC-BIOTECHNOLOGY & BIOLOGICAL SCIENCES RESEARCH COUNCIL; BB/R014817/1

How to connect an eye to a brain; G3137; WELLCOME TRUST; WT Ref: 220277/

Philip Leverhulme Prize - Biological Sciences; G2276; LEVERHULME TRUST; PLP-2017-005

Anisotropic retinal circuits for processing of colour and space in nature - Lister Institute Research Prize; G2503; LISTER INSTITUTE

NeuroVisEco - Zebrafish vision in its natural context: from natural scenes through retinal and central processing to behaviour; G1871; EUROPEAN UNION; 677687

EMBO Young Investigator Programme; G2920; EMBO-EUROPEAN MOLECULAR BIOLOGY ORGANIZATION; Baden

History

Publication status

  • Published

File Version

  • Published version

Journal

Nature Communications

ISSN

2041-1723

Publisher

Nature Research

Issue

1

Volume

13

Page range

1-20

Article number

a544

Department affiliated with

  • Neuroscience Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2022-02-03

First Open Access (FOA) Date

2022-02-03

First Compliant Deposit (FCD) Date

2022-02-02

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