SK current, expressed during the development and regeneration of chick hair cells, contributes to the patterning of spontaneous action potentials

Levic, Snezana (2022) SK current, expressed during the development and regeneration of chick hair cells, contributes to the patterning of spontaneous action potentials. Frontiers in Cellular Neuroscience, 15. a766264 1-14. ISSN 1662-5102

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Abstract

Chick hair cells display calcium (Ca2+)-sensitive spontaneous action potentials during development and regeneration. The role of this activity is unclear but thought to be involved in establishing proper synaptic connections and tonotopic maps, both of which are instrumental to normal hearing. Using an electrophysiological approach, this work investigated the functional expression of Ca2+-sensitive potassium [IK(Ca)] currents and their role in spontaneous electrical activity in the developing and regenerating hair cells (HCs) in the chick basilar papilla. The main IK(Ca) in developing and regenerating chick HCs is an SK current, based on its sensitivity to apamin. Analysis of the functional expression of SK current showed that most dramatic changes occurred between E8 and E16. Specifically, there is a developmental downregulation of the SK current after E16. The SK current gating was very sensitive to the availability of intracellular Ca2+ but showed very little sensitivity to T-type voltage-gated Ca2+ channels, which are one of the hallmarks of developing and regenerating hair cells. Additionally, apamin reduced the frequency of spontaneous electrical activity in HCs, suggesting that SK current participates in patterning the spontaneous electrical activity of HCs.

Item Type: Article
Schools and Departments: Brighton and Sussex Medical School > Neuroscience
SWORD Depositor: Mx Elements Account
Depositing User: Mx Elements Account
Date Deposited: 25 Jan 2022 11:11
Last Modified: 03 Mar 2022 13:29
URI: http://sro.sussex.ac.uk/id/eprint/103943

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Project NameSussex Project NumberFunderFunder Ref
Interaction between sensory and supporting cells in the organ of Corti: basis for sensitivity and frequency selectivity of mammalian cochlea.G1832MRC-MEDICAL RESEARCH COUNCILMR/N004299/1