2021 - Paper, Journal of Material Chemistry B(Graphene-GBM).pdf (5.83 MB)
Tuneable synthetic reduced graphene oxide scaffolds elicit high levels of three-dimensional glioblastoma interconnectivity in vitro
journal contribution
posted on 2023-06-10, 02:19 authored by Christopher BrownChristopher Brown, Thomas Simon, Chiara Cilibrasi, Peter LynchPeter Lynch, Rhiannon Harries, Aline Amorim GrafAline Amorim Graf, Matthew LargeMatthew Large, Sean OgilvieSean Ogilvie, Jonathan P Salvage, Alan DaltonAlan Dalton, Georgios GiamasGeorgios Giamas, Alice KingAlice KingThree-dimensional tissue scaffolds have utilised nanomaterials to great effect over the last decade. In particular, scaffold design has evolved to consider mechanical structure, morphology, chemistry, electrical properties, and of course biocompatibility - all vital to the performance of the scaffold and how successful they are in developing cell cultures. We have developed an entirely synthetic and tuneable three-dimensional scaffold of reduced graphene oxide (rGO) that shows good biocompatibility, and favourable mechanical properties as well as reasonable electrical conductivity. Importantly, the synthesis is scaleable and suitable for producing scaffolds of any desired geometry and size, and we observe a high level of biocompatibility and cell proliferation for multiple cell lines. In particular, one of the most devastating forms of malignant brain cancer, glioblastoma (GBM), grows especially well on our rGO scaffold in vitro, and without the addition of response-specific growth factors. We have observed that our scaffold elicits spontaneous formation of a high degree of intercellular connections across the GBM culture. This phenomenon is not well documented in vitro and nothing similar has been observed in synthetic scaffolds without the use of response-specific growth factors - which risk obscuring any potential phenotypic behaviour of the cells. The use of scaffolds like ours, which are not subject to the limitations of existing two-dimensional substrate technologies, provide an excellent system for further investigation into the mechanisms behind the rapid proliferation and success of cancers like GBM. These synthetic scaffolds can advance our understanding of these malignancies in the pursuit of improved theranostics against them.
History
Publication status
- Published
File Version
- Published version
Journal
Journal of Materials Chemistry BISSN
2050-750XPublisher
Royal Society of ChemistryExternal DOI
Issue
30Volume
10Page range
373-383Event location
EnglandDepartment affiliated with
- Biochemistry Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2022-01-18First Open Access (FOA) Date
2022-01-18First Compliant Deposit (FCD) Date
2022-01-17Usage metrics
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