Cross-neutralisation of novel Bombali virus by Ebola virus antibodies and convalescent plasma using an optimised pseudotype-based neutralisation assay

Bentley, Emma M, Richardson, Samuel, Derveni, Mariliza, Rijal, Pramila, Townsend, Alain R, Heeney, Jonathan L, Mattiuzzo, Giada and Wright, Edward (2021) Cross-neutralisation of novel Bombali virus by Ebola virus antibodies and convalescent plasma using an optimised pseudotype-based neutralisation assay. Tropical Medicine and Infectious Disease, 6 (3). a155 1-12. ISSN 2414-6366

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Abstract

Ebolaviruses continue to pose a significant outbreak threat, and while Ebola virus (EBOV)- specific vaccines and antivirals have been licensed, efforts to develop candidates offering broad species cross-protection are continuing. The use of pseudotyped virus in place of live virus is recognised as an alternative, safer, high-throughput platform to evaluate anti-ebolavirus antibodies towards their development, yet it requires optimisation. Here, we have shown that the target cell line impacts neutralisation assay results and cannot be selected purely based on permissiveness. In expanding the platform to incorporate each of the ebolavirus species envelope glycoprotein, allowing a comprehensive assessment of cross-neutralisation, we found that the recently discovered Bombali virus has a point mutation in the receptor-binding domain which prevents entry into a hamster cell line and, importantly, shows that this virus can be cross-neutralised by EBOV antibodies and convalescent plasma.

Item Type: Article
Keywords: cross-neutralisation, ebolavirus, pseudotyped virus
Schools and Departments: School of Life Sciences > Biochemistry
SWORD Depositor: Mx Elements Account
Depositing User: Mx Elements Account
Date Deposited: 14 Jan 2022 12:36
Last Modified: 14 Jan 2022 12:45
URI: http://sro.sussex.ac.uk/id/eprint/103827

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