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Cross-neutralisation of novel Bombali virus by Ebola virus antibodies and convalescent plasma using an optimised pseudotype-based neutralisation assay

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posted on 2023-06-10, 02:17 authored by Emma M Bentley, Samuel Richardson, Maria Derveni, Pramila Rijal, Alain R Townsend, Jonathan L Heeney, Giada Mattiuzzo, Edward WrightEdward Wright
Ebolaviruses continue to pose a significant outbreak threat, and while Ebola virus (EBOV)- specific vaccines and antivirals have been licensed, efforts to develop candidates offering broad species cross-protection are continuing. The use of pseudotyped virus in place of live virus is recognised as an alternative, safer, high-throughput platform to evaluate anti-ebolavirus antibodies towards their development, yet it requires optimisation. Here, we have shown that the target cell line impacts neutralisation assay results and cannot be selected purely based on permissiveness. In expanding the platform to incorporate each of the ebolavirus species envelope glycoprotein, allowing a comprehensive assessment of cross-neutralisation, we found that the recently discovered Bombali virus has a point mutation in the receptor-binding domain which prevents entry into a hamster cell line and, importantly, shows that this virus can be cross-neutralised by EBOV antibodies and convalescent plasma.

History

Publication status

  • Published

File Version

  • Published version

Journal

Tropical Medicine and Infectious Disease

ISSN

2414-6366

Publisher

MDPI

Issue

3

Volume

6

Page range

1-12

Article number

a155

Event location

Switzerland

Department affiliated with

  • Biochemistry Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2022-01-14

First Open Access (FOA) Date

2022-01-14

First Compliant Deposit (FCD) Date

2022-01-14

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