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Whole genome analysis and functional characterization of a novel Bacillus thuringiensis (Bt 62) isolate against sugarcane white grub Holotrichia serrata (F)
journal contribution
posted on 2023-06-10, 02:11 authored by M Naveenarani, G S Suresha, J Srikanth, K Hari, C Sankaranarayanan, P Mahesh, R Nirmala, C P Swathik, Neil CrickmoreNeil Crickmore, B Ram, C Appunu, B SingaraveluIn this study, we report the whole genome assembly of Bt 62, a novel isolate harbouring cry8 holotype gene identified by us earlier. Sequencing was carried out using a combination of Illumina NextSeq 500 and Oxford Nanopore sequencing Technologies (ONT). The final assembled genome was 6.13 Mb comprising a circular chromosome and four plasmids. The bioassay studies against Holotrichia serrata (F.) (Coleoptera: Scarabaeidae), a polyphagous pest infesting sugarcane and other crops, indicated significant toxicity to first instar grubs over untreated larvae achieving a highest mean mortality of 91.11% for various doses tested. In vitro proteolytic assay and histopathological studies of the midgut of infected white grubs revealed proteolytic processing of the protoxin and extensive degeneration of larval midgut epithelial cells. The results demonstrate that this novel isolate could be used as a biopesticide or its crystal toxin genes could be expressed in sugarcane and other crops for resistance against H. serrata.
History
Publication status
- Published
File Version
- Published version
Journal
GenomicsISSN
0888-7543Publisher
ElsevierExternal DOI
Issue
1Volume
114Page range
185-195Event location
United StatesDepartment affiliated with
- Biochemistry Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2022-01-06First Open Access (FOA) Date
2022-01-06First Compliant Deposit (FCD) Date
2022-01-06Usage metrics
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