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Transition of nano-architectures through self-assembly of lipidated ß3-tripeptide foldamers

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posted on 2023-06-10, 00:59 authored by Nathan Habila, Ketav Kulkarni, Tzong-Hsien Lee, Zahraa S Al-Garawi, Louise SerpellLouise Serpell, Marie-Isabel Aguilar, Mark P Del Borgo
ß3-peptides consisting exclusively of ß3-amino acids adopt a variety of non-natural helical structures and can self-assemble into well-defined hierarchical structures by axial head-to-tail self-assembly resulting in fibrous materials of varying sizes and shapes. To allow control of fiber morphology, a lipid moiety was introduced within a tri-ß3-peptide sequence at each of the three amino acid positions and the N-terminus to gain finer control over the lateral assembly of fibers. Depending on the position of the lipid, the self-assembled structures formed either twisted ribbon-like fibers or distinctive multilaminar nanobelts. The nanobelt structures were comprised of multiple layers of peptide fibrils as revealed by puncturing the surface of the nanobelts with an AFM probe. This stacking phenomenon was completely inhibited through changes in pH, indicating that the layer stacking was mediated by electrostatic interactions. Thus, the present study is the first to show controlled self-assembly of these fibrous structures, which is governed by the location of the acyl chain in combination with the 3-point H-bonding motif. Overall, the results demonstrate that the nanostructures formed by the ß3-tripeptide foldamers can be tuned via sequential lipidation of N-acetyl ß3-tripeptides which control the lateral interactions between peptide fibrils and provide defined structures with a greater homogeneous population.

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Publication status

  • Published

File Version

  • Published version

Journal

Frontiers in Chemistry

ISSN

2296-2646

Publisher

Frontiers

Volume

8

Page range

217

Event location

Switzerland

Department affiliated with

  • Neuroscience Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2021-09-17

First Open Access (FOA) Date

2021-09-17

First Compliant Deposit (FCD) Date

2021-09-17

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