Mirzaeei2021_Article_PolyvinylAlcoholChitosanSingle.pdf (2.93 MB)
Polyvinyl Alcohol/Chitosan single-layered and Polyvinyl Alcohol/Chitosan/Eudragit RL100 multi-layered electrospun nanofibers as an ocular matrix for the controlled release of ofloxacin: an in vitro and in vivo evaluation
journal contribution
posted on 2023-06-10, 00:56 authored by Shahla Mirzaeei, Shiva Taghe, Kofi Asare-Addo, Ali NokhodchiA novel nanofiber insert was prepared with a modified electrospinning method to enhance the ocular residence time of ofloxacin (OFX) and to provide a sustained release pattern by covering hydrophilic polymers, chitosan/polyvinyl alcohol (CS/PVA) nanofibers, with a hydrophobic polymer, Eudragit RL100 in layers, and by glutaraldehyde (GA) cross-linking of CS-PVA nanofibers for the treatment of infectious conjunctivitis. The morphology of the prepared nanofibers was studied using scanning electron microscopy (SEM). The average fiber diameter was found to be 123 ± 23 nm for the single electrospun nanofiber with no cross-linking (OFX-O). The single nanofibers, cross-linked for 10 h with GA (OFX-OG), had an average fiber diameter of 159 ± 30 nm. The amount of OFX released from the nanofibers was measured in vitro and in vivo using UV spectroscopy and microbial assay methods against Staphylococcus aureus, respectively. The antimicrobial efficiency of OFX formulated in cross-linked and non-cross-linked nanofibers was affirmed by observing the inhibition zones of Staphylococcus aureus and Escherichia coli. In vivo studies using the OFX nanofibrous inserts on a rabbit eye confirmed a sustained release pattern for up to 96 h. It was found that the cross-linking of the nanofibers by GA vapor could reduce the burst release of OFX from OFX-loaded CS/PVA in one layer and multi-layered nanofibers. In vivo results showed that the AUC0–96 for the nanofibers was 9–20-folds higher compared to the OFX solution. This study thus demonstrates the potential of the nanofiber technology is being utilized to sustained drug release in ocular drug delivery systems.
History
Publication status
- Published
File Version
- Published version
Journal
AAPS PharmSciTechISSN
1530-9932Publisher
SpringerExternal DOI
Issue
5Volume
22Page range
1-13Article number
a170Event location
United StatesDepartment affiliated with
- Chemistry Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2021-09-10First Open Access (FOA) Date
2021-09-10First Compliant Deposit (FCD) Date
2021-09-10Usage metrics
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No categories selectedKeywords
Eudragit RL100chitosannanofiberocular drug deliverysustained release insertAcrylic ResinsAdministrationOphthalmicAnimalsAnti-Bacterial AgentsChemistryPharmaceuticalChitosanDelayed-Action PreparationsDrug Delivery SystemsDrug EvaluationPreclinicalEscherichia coliNanofibersOfloxacinPolyvinyl AlcoholRabbitsStaphylococcus aureus
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