Wu, Tianyu, Lane, Simon I R, Morgan, Stephanie L, Tang, Feng and Jones, Keith T (2021) Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I. Journal of Cell Biology, 220 (10). ISSN 0021-9525

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Abstract

The repetitive sequences of DNA centromeric regions form the structural basis for kinetochore assembly. Recently they were found to be transcriptionally active in mitosis, with their RNAs providing non-coding functions. Here we explore the role, in mouse oocytes, of transcripts generated from within the minor satellite repeats. Depletion of minor satellite transcripts delayed progression through meiosis I by activation of the spindle assembly checkpoint. Arrested oocytes had poorly congressed chromosomes and centromeres were frequently split by microtubules. Thus, we have demonstrated that the centromeric RNA plays a specific role in female meiosis I compared to mitosis and is required for maintaining the structural integrity of centromeres. This may contribute to the high aneuploidy rates observed in female meiosis.

Item Type:	Article
Schools and Departments:	School of Life Sciences > Biochemistry Professional Services > Vice-Chancellor's Office
SWORD Depositor:	Mx Elements Account
Depositing User:	Mx Elements Account
Date Deposited:	29 Jul 2021 07:06
Last Modified:	11 Aug 2021 15:45
URI:	http://sro.sussex.ac.uk/id/eprint/100828

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