Spink, K, Ho, J C, Tanaka, K, Watts, Felicity and Chambers, Anna (2005) The telomere-binding protein Taz1p as a target for modification by a SUMO-1 homologue in fission yeast. Biochemical Genetics, 43 (3-4). pp. 103-17. ISSN 0006-2928Full text not available from this repository.
In fission yeast (Schizosaccharomyces pombe) the homologue of the mammalian SUMO-1 ubiquitin-like modifier is encoded by the pmt3 gene. A two-hybrid screen using the telomere-binding protein Taz1p as bait identified Pmt3p as an interacting factor. In vitro experiments using purified components of the fission yeast Pmt3p modification system demonstrated that Taz1p could be modified directly by Pmt3p. The amino acid sequence of Taz1p contains a close match to the consensus modification site for SUMO-1, and a PEST sequence similar to those found in established SUMO-1 targets. Although previous experiments have identified an increase in telomere length as one consequence of the pmt3--genotype, we could not detect Pmt3p modification of Taz1p in protein extracts made from exponentially growing haploid cells or any effect of Pmt3p on the localization of GFP-Taz1p at discrete foci in the haploid cell nucleus.
|Keywords:||Repressor Proteins/*metabolism Research Support, Non-U.S. Gov't Schizosaccharomyces/genetics/metabolism Schizosaccharomyces pombe Proteins/*metabolism Telomere-Binding Proteins/*metabolism Two-Hybrid System Techniques Ubiquitin-Activating Enzymes/*metabolism|
|Schools and Departments:||School of Life Sciences > Sussex Centre for Genome Damage and Stability|
|Depositing User:||Gee Wheatley|
|Date Deposited:||19 Mar 2007|
|Last Modified:||25 Jun 2015 10:43|
|Google Scholar:||7 Citations|