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Quantification of global transcription patterns in prokaryotes using spotted microarrays

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posted on 2023-06-07, 16:22 authored by Ben Sidders, Mike Withers, Sharon L Kendall, Joanna Bacon, Simon WaddellSimon Waddell, Jason Hinds, Paul Golby, Farahnaz Movahedzadeh, Robert A Cox, Rosangela Frita, Annemieke M C Ten Bokum, Lorenz Wernisch, Neil G Stoker
We describe an analysis, applicable to any spotted microarray dataset produced using genomic DNA as a reference, that quantifies prokaryotic levels of mRNA on a genome-wide scale. Applying this to Mycobacterium tuberculosis, we validate the technique, show a correlation between level of expression and biological importance, define the complement of invariant genes and analyze absolute levels of expression by functional class to develop ways of understanding an organism's biology without comparison to another growth condition.

History

Publication status

  • Published

File Version

  • Published version

Journal

Genome Biology

ISSN

1465-6906

Publisher

BioMed Central

Issue

12

Volume

8

Page range

R265

Department affiliated with

  • Global Health and Infection Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2012-07-17

First Open Access (FOA) Date

2017-01-10

First Compliant Deposit (FCD) Date

2017-01-10

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