Quantification of global transcription patterns in prokaryotes using spotted microarrays

Sidders, Ben, Withers, Mike, Kendall, Sharon L, Bacon, Joanna, Waddell, Simon J, Hinds, Jason, Golby, Paul, Movahedzadeh, Farahnaz, Cox, Robert A, Frita, Rosangela, Ten Bokum, Annemieke M C, Wernisch, Lorenz and Stoker, Neil G (2007) Quantification of global transcription patterns in prokaryotes using spotted microarrays. Genome Biology, 8 (12). R265. ISSN 1465-6906

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Abstract

We describe an analysis, applicable to any spotted microarray dataset produced using genomic DNA as a reference, that quantifies prokaryotic levels of mRNA on a genome-wide scale. Applying this to Mycobacterium tuberculosis, we validate the technique, show a correlation between level of expression and biological importance, define the complement of invariant genes and analyze absolute levels of expression by functional class to develop ways of understanding an organism's biology without comparison to another growth condition.

Item Type: Article
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology > QR0075 Bacteria
Depositing User: Simon Waddell
Date Deposited: 17 Jul 2012 14:41
Last Modified: 07 Mar 2017 06:45
URI: http://sro.sussex.ac.uk/id/eprint/7634

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