The use of microarray analysis to determine the gene expression profiles of Mycobacterium tuberculosis in response to anti-bacterial compounds

Waddell, Simon J, Stabler, Richard A, Laing, Ken, Kremer, Laurent, Reynolds, Robert C and Besra, Gurdyal S (2004) The use of microarray analysis to determine the gene expression profiles of Mycobacterium tuberculosis in response to anti-bacterial compounds. Tuberculosis, 84 (3-4). pp. 263-274. ISSN 1472-9792

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Abstract

The response of Mycobacterium tuberculosis to six anti-microbial agents was determined by microarray analysis in an attempt to define mechanisms of innate resistance in M. tuberculosis. The gene expression profiles of M. tuberculosis after treatment at the minimal inhibitory concentration (MIC) for 4 h with isoniazid, isoxyl, tetrahydrolipstatin, SRI#221, SR1#967 and SR1#9190 were compared to untreated M. tuberculosis. A common response to drug exposure was defined, and this expression profile overlapped with a number of other mycobacterial stress responses recently identified by microarray analysis. Compound-specific responses were also distinguished including a number of putative transcriptional regulators and translocation-related genes. These genes may contribute to the intrinsic resistance of M. tuberculosis to anti-microbial compounds. Further investigation into these mechanisms may elucidate novel pathways contributing to mycobacterial drug resistance and influence anti-mycobacterial drug development strategies.

Item Type: Article
Keywords: Mycobacterium tuberculosis; Innate drug resistance; Microarray; Isoniazid; Isoxyl; Tetrahydrolipstatin
Schools and Departments: Brighton and Sussex Medical School > Global Health and Infection
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology > QR0075 Bacteria
Depositing User: Simon Waddell
Date Deposited: 17 Jul 2012 14:19
Last Modified: 22 Sep 2017 11:24
URI: http://sro.sussex.ac.uk/id/eprint/7630

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