Ubiquitin-family modifications in the replication of DNA damage

Lehmann, Alan R (2011) Ubiquitin-family modifications in the replication of DNA damage. FEBS Letters, 585 (18). pp. 2772-2779. ISSN 0014-5793

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Abstract

The cell uses specialised Y-family DNA polymerases or damage avoidance mechanisms to replicate past damaged sites in DNA. These processes are under complex regulatory systems, which employ different types of post-translational modification. All the Y-family polymerases have ubiquitin binding domains that bind to mono-ubiquitinated PCNA to effect the switching from replicative to Yfamily polymerase. Ubiquitination and de-ubiquitination of PCNA are tightly regulated. There is also evidence for another as yet unidentified ubiquitinated protein being involved in recruitment of Yfamily polymerases to chromatin. Poly-ubiquitination of PCNA stimulates damage avoidance, and, at least in yeast, PCNA is SUMOylated to prevent unwanted recombination events at the replication fork. The Y-family polymerases themselves can be ubiquitinated and, in the case of DNA polymerase g, this results in the polymerase being excluded from chromatin.

Item Type: Article
Keywords: DNA polymerase; PCNA; UV light
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science
Depositing User: Philippa Erasmus
Date Deposited: 09 Dec 2011 12:39
Last Modified: 13 Mar 2017 10:44
URI: http://sro.sussex.ac.uk/id/eprint/7498

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