A cytochrome P450 family 2 subfamily R member 1 gene variant determines response to vitamin D after 12 weeks supplementation

Barami, A, Mehramiz, M, Ghayour-Mobarhan, M, Bahrami-Taghanaki, H, Ferns, G, Sadeghnia, HR and Avan, A (2018) A cytochrome P450 family 2 subfamily R member 1 gene variant determines response to vitamin D after 12 weeks supplementation. Clinical Nutrition. ISSN 0261-5614 (Accepted)

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Abstract

Background: Globally, about 1 billion people have inadequate levels of serum vitamin D and it appears it is prevalent in all ethnicities and age groups. Few foods naturally contain enough vitamin D; therefore, most people get their need through supplementation. Response to supplementation is modulated by both genetic and environmental determinants including season of measurement, diet habitual, health status, body mass index and medication. It is clear that genetic epidemiology of vitamin D is in its infancy step and further studies would be needed to understand on how genetic variation influences vitamin D metabolism. We aimed to explore association of vitamin D-related polymorphism rs10766197 in CYP2R1 gene with response to supplementation of vitamin D in 253 healthy Iranian girls.
Material and method: A total of 253 healthy subjects received 50000 IU vitamin D3 monthly for 12 months. Serum 25(OH)D concentration and metabolic profiles were measured at baseline and after 12 months of supplementation. CYP2R1 variant (rs10766197) were identified using TaqMan genotyping assays.
Results: Our data showed that serum 25(OH)D during the supplementation, increased in all groups. Interestingly subjects with allele G had higher vitamin D concentrations after intervention. Also our results revealed that this genetic variant modulated response to intervention (p-value of intervention effect <0.001 and p-value SNP=0.05). The regression analysis also indicates that probability of changing in serum 25(OH)D, in individuals who had homozygous major allele GG was twice more than carriers of the rare allele A (OR=2.1 (1-4.2); p value=0.03). Further analysis illustrated that fast blood glucose and triglyceride concentration alleviated in carries of GG genotype. Interestingly, the inflammation status also was ameliorated in AA carries while the in individuals with GG and AG genotypes, after vitamin D supplementation.
Conclusion: Improvements in serum vitamin D and metabolic profile due to vitamin D supplementation were associated with CYP2R1 polymorphism. Although carriers of the dominant G allele showed more improvement in concentrations of vitamin D.

Item Type: Article
Keywords: 25(OH)D, Supplementation, CYP1R2, rs10766197
Schools and Departments: Brighton and Sussex Medical School > Division of Medical Education
Depositing User: Gordon Ferns
Date Deposited: 04 Apr 2018 14:09
Last Modified: 21 Sep 2018 07:30
URI: http://sro.sussex.ac.uk/id/eprint/74787

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