A CREB2-targeting microRNA is required for long-term memory after single-trial learning

Korneev, Sergei A, Vavoulis, Dimitris V, Naskar, Souvik, Dyakonova, Varvara E, Kemenes, Ildikó and Kemenes, György (2018) A CREB2-targeting microRNA is required for long-term memory after single-trial learning. Scientific Reports, 8 (8950). ISSN 2045-2322

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Abstract

Although single-trial induced long-term memories (LTM) have been of major interest in neuroscience, how LTM can form after a single episode of learning remains largely unknown. We hypothesized that the removal of molecular inhibitory constraints by microRNAs (miRNAs) plays an important role in this process. To test this hypothesis, first we constructed small non-coding RNA (sncRNA) cDNA libraries from the CNS of Lymnaea stagnalis subjected to a single conditioning trial. Then, by next generation sequencing of these libraries, we identified a specific pool of miRNAs regulated by training. Of these miRNAs, we focussed on Lym-miR-137 whose seed region shows perfect complementarity to a target sequence in the 3’ UTR of the mRNA for CREB2, a well-known memory repressor. We found that Lym-miR-137 was transiently up-regulated 1 h after single-trial conditioning, preceding a down-regulation of Lym-CREB2 mRNA. Furthermore, we discovered that Lym-miR-137 is co-expressed with Lym-CREB2 mRNA in an identified neuron with an established role in LTM. Finally, using an in vivo loss-of-function approach we demonstrated that Lym-miR-137 is required for single-trial induced LTM.

Item Type: Article
Keywords: Memory formation Non-coding RNAs MicroRNAs CREB
Schools and Departments: School of Life Sciences > Neuroscience
Research Centres and Groups: Sussex Neuroscience
Depositing User: Sergei Korneev
Date Deposited: 20 Feb 2018 14:19
Last Modified: 21 May 2018 15:51
URI: http://sro.sussex.ac.uk/id/eprint/73736

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