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The time course of ongoing activity during neuritis and following axonal transport disruption

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posted on 2023-06-09, 12:16 authored by Ieva Satkeviciute, George Goodwin, Geoffrey M Bove, Andrew DilleyAndrew Dilley
Local nerve inflammation (neuritis) leads to ongoing activity and axonal mechanical sensitivity (AMS) along intact nociceptor axons, and disrupts axonal transport. This phenomenon forms the most feasible cause of radiating pain, such as sciatica. We have previously shown that axonal transport disruption without inflammation or degeneration also leads to AMS, but does not cause ongoing activity at the time point when AMS occurs, despite causing cutaneous hypersensitivity. However, there have been no systematic studies of ongoing activity during neuritis or non-inflammatory axonal transport disruption. In this study, we present the time course of ongoing activity from primary sensory neurons following neuritis and vinblastine-induced axonal transport disruption. Whereas 24% of C/slow Ad-fiber neurons had ongoing activity during neuritis, few (<10%) A- and C-fiber neurons showed ongoing activity 1-15 days following vinblastine treatment. In contrast, AMS increased transiently at the vinblastine treatment site, peaking on day 4-5 (28% of C/slow Ad-fiber neurons) and resolved by day 15. Conduction velocities were slowed in all groups. In summary, the disruption of axonal transport without inflammation does not lead to ongoing activity in sensory neurons, including nociceptors, but does cause a rapid and transient development of AMS. Since it is proposed that AMS underlies mechanically-induced radiating pain, and a transient disruption of axonal transport (as previously reported) leads to transient AMS, it follows that processes that disrupt axonal transport, such as neuritis, must persist to maintain AMS and the associated symptoms.

Funding

NC3Rs; NC/L00156X/1

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Journal of Neurophysiology

ISSN

0022-3077

Publisher

American Physiological Society

Issue

5

Volume

119

Page range

1993-2000

Department affiliated with

  • BSMS Neuroscience Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2018-02-20

First Open Access (FOA) Date

2019-05-01

First Compliant Deposit (FCD) Date

2018-02-20

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