Schizosaccharomyces pombe MutSα and MutLα maintain stability of tetra-nucleotide repeats and Msh3 of hepta-nucleotide repeats

Villahermosa, Desirée, Christensen, Olaf, Knapp, Karen and Fleck, Oliver (2017) Schizosaccharomyces pombe MutSα and MutLα maintain stability of tetra-nucleotide repeats and Msh3 of hepta-nucleotide repeats. G3, 7 (5). pp. 1463-1473. ISSN 2160-1836

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Abstract

Defective mismatch repair (MMR) in humans is associated with colon cancer and instability of microsatellites, that is, DNA sequences with one or several nucleotides repeated. Key factors of eukaryotic MMR are the heterodimers MutSa (Msh2-Msh6), which recognizes base-base mismatches and unpaired nucleotides in DNA, and MutLa (Mlh1-Pms1), which facilitates downstream steps. In addition, MutSb (Msh2- Msh3) recognizes DNA loops of various sizes, although our previous data and the data presented here suggest that Msh3 of Schizosaccharomyces pombe does not play a role in MMR. To test microsatellite stability in S. pombe and hence DNA loop repair, we have inserted tetra-, penta-, and hepta-nucleotide repeats in the ade6 gene and determined their Ade+ reversion rates and spectra in wild type and various mutants. Our data indicate that loops with four unpaired nucleotides in the nascent and the template strand are the upper limit of MutSa- and MutLa-mediated MMR in S. pombe. Stability of hepta-nucleotide repeats requires Msh3 and Exo1 in MMR-independent processes as well as the DNA repair proteins Rad50, Rad51, and Rad2FEN1. Most strikingly, mutation rates in the double mutants msh3 exo1 and msh3 rad51 were decreased when compared to respective single mutants, indicating that Msh3 prevents error prone processes carried out by Exo1 and Rad51. We conclude that Msh3 has no obvious function in MMR in S. pombe, but contributes to DNA repeat stability in MMR-independent processes.

Item Type: Article
Keywords: mismatch repair microsatellite instability homologous recombination repair repetitive DNA FEN1
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Research Centres and Groups: Genome Damage and Stability Centre
Depositing User: Dr Desiree Villahermosa Caballero
Date Deposited: 06 Feb 2018 11:26
Last Modified: 06 Feb 2018 11:26
URI: http://sro.sussex.ac.uk/id/eprint/73366

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