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Primary liver cells cultured on carbon nanotube substrates for liver tissue engineering and drug discovery applications
journal contribution
posted on 2023-06-09, 11:41 authored by Che Azurahanim Che Abdullah, Chihye Lewes Azad, Raquel Ovalle-Robles, Shaoli Fang, Marcio D Lima, Xavier Lepró, Steve Collins, Ray H Baughman, Alan DaltonAlan Dalton, Nick J Plant, Richard P SearHere, we explore the use of two- and three-dimensional scaffolds of multiwalled-carbon nanotubes (MWNTs) for hepatocyte cell culture. Our objective is to study the use of these scaffolds in liver tissue engineering and drug discovery. In our experiments, primary rat hepatocytes, the parenchymal (main functional) cell type in the liver, were cultured on aligned nanogrooved MWNT sheets, MWNT yarns, or standard 2-dimensional culture conditions as a control. We find comparable cell viability between all three culture conditions but enhanced production of the hepatocytespecific marker albumin for cells cultured on MWNTs. The basal activity of two clinically relevant cytochrome P450 enzymes, CYP1A2 and CYP3A4, are similar on all substrates, but we find enhanced induction of CYP1A2 for cells on the MWNT sheets. Our data thus supports the use of these substrates for applications including tissue engineering and enhancing liver-specific functions, as well as in in vitro model systems with enhanced predictive capability in drug discovery and development.
History
Publication status
- Published
File Version
- Published version
Journal
ACS Applied Materials and InterfacesISSN
1944-8244Publisher
American Chemical SocietyExternal DOI
Issue
13Volume
6Page range
10373-10380Department affiliated with
- Physics and Astronomy Publications
Research groups affiliated with
- Materials Physics Group Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2018-01-19First Compliant Deposit (FCD) Date
2018-01-19Usage metrics
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