Primary liver cells cultured on carbon nanotube substrates for liver tissue engineering and drug discovery applications

Abdullah, Che Azurahanim Che, Azad, Chihye Lewes, Ovalle-Robles, Raquel, Fang, Shaoli, Lima, Marcio D, Lepró, Xavier, Collins, Steve, Baughman, Ray H, Dalton, Alan, Plant, Nick J and Sear, Richard P (2014) Primary liver cells cultured on carbon nanotube substrates for liver tissue engineering and drug discovery applications. ACS Applied Materials and Interfaces, 6 (13). pp. 10373-10380. ISSN 1944-8244

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Abstract

Here, we explore the use of two- and three-dimensional scaffolds of multiwalled-carbon nanotubes (MWNTs) for hepatocyte cell culture. Our objective is to study the use of these scaffolds in liver tissue engineering and drug discovery. In our experiments, primary rat hepatocytes, the parenchymal (main functional) cell type in the liver, were cultured on aligned nanogrooved MWNT sheets, MWNT yarns, or standard 2-dimensional culture conditions as a control. We find comparable cell viability between all three culture conditions but enhanced production of the hepatocytespecific marker albumin for cells cultured on MWNTs. The basal activity of two clinically relevant cytochrome P450 enzymes, CYP1A2 and CYP3A4, are similar on all substrates, but we find enhanced induction of CYP1A2 for cells on the MWNT sheets. Our data thus supports the use of these substrates for applications including tissue engineering and enhancing liver-specific functions, as well as in in vitro model systems with enhanced predictive capability in drug discovery and development.

Item Type: Article
Schools and Departments: School of Mathematical and Physical Sciences > Physics and Astronomy
Research Centres and Groups: Materials Physics Group
Subjects: Q Science
Depositing User: Alan Dalton
Date Deposited: 19 Jan 2018 11:17
Last Modified: 25 Jan 2018 09:45
URI: http://sro.sussex.ac.uk/id/eprint/72979

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