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Primary liver cells cultured on carbon nanotube substrates for liver tissue engineering and drug discovery applications

journal contribution
posted on 2023-06-09, 11:41 authored by Che Azurahanim Che Abdullah, Chihye Lewes Azad, Raquel Ovalle-Robles, Shaoli Fang, Marcio D Lima, Xavier Lepró, Steve Collins, Ray H Baughman, Alan DaltonAlan Dalton, Nick J Plant, Richard P Sear
Here, we explore the use of two- and three-dimensional scaffolds of multiwalled-carbon nanotubes (MWNTs) for hepatocyte cell culture. Our objective is to study the use of these scaffolds in liver tissue engineering and drug discovery. In our experiments, primary rat hepatocytes, the parenchymal (main functional) cell type in the liver, were cultured on aligned nanogrooved MWNT sheets, MWNT yarns, or standard 2-dimensional culture conditions as a control. We find comparable cell viability between all three culture conditions but enhanced production of the hepatocytespecific marker albumin for cells cultured on MWNTs. The basal activity of two clinically relevant cytochrome P450 enzymes, CYP1A2 and CYP3A4, are similar on all substrates, but we find enhanced induction of CYP1A2 for cells on the MWNT sheets. Our data thus supports the use of these substrates for applications including tissue engineering and enhancing liver-specific functions, as well as in in vitro model systems with enhanced predictive capability in drug discovery and development.

History

Publication status

  • Published

File Version

  • Published version

Journal

ACS Applied Materials and Interfaces

ISSN

1944-8244

Publisher

American Chemical Society

Issue

13

Volume

6

Page range

10373-10380

Department affiliated with

  • Physics and Astronomy Publications

Research groups affiliated with

  • Materials Physics Group Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2018-01-19

First Compliant Deposit (FCD) Date

2018-01-19

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