Williams, Rebecca, Nelson, Larissa, Dowthwaite, Gary P., Evans, Darrell J.R. and Archer, Charles W. (2009) Notch receptor and Notch ligand expression in developing avian cartilage. Journal of Anatomy, 215 (2). pp. 159-169. ISSN 0021-8782Full text not available from this repository.
The development of limb cartilage involves complex signalling pathways allowing the formation of distinct segments of cartilage that are maintained in the fully developed joint. In this study, we investigated the Notch signalling pathway and its role in cartilage development. The differential distribution of the Notch signalling family of receptors and their corresponding ligands in developing avian (gallus gallus) cartilage revealed expression of Notch 1, Delta 1, Jagged 1 and Jagged 2 in all limb mesenchyme cells at the early stages of cartilage anlagen development, which were subsequently restricted to the developing cartilage element. Expression of both Notch 1 and Jagged 1 became increasingly restricted to the surface cartilage once joint cavity formation had occurred. Delta 1 and Jagged 1 were restricted to a layer of cells underneath the surface cartilage and were also observed in the hypertrophic chondrocytes, where Notch 1 expression was evident in stage 40-44 limbs. Notch 2, Notch 3 and Notch 4 were not evident in early stage limbs but were present after cavitation, although expression was lost in late stage limbs (stage 40-44). We also demonstrated that inhibition of the Notch pathway leads to altered Notch receptor expression, disrupting cartilage differentiation. From these data it is clear that Notch signalling is a necessary and critical factor in regulating cell fate decisions allowing controlled chondrogenesis, elongation and subsequent maintenance of limb cartilage.
|Schools and Departments:||Brighton and Sussex Medical School > Clinical and Laboratory Investigation|
|Subjects:||Q Science > QH Natural history > QH0301 Biology
Q Science > QL Zoology > QL0799 Morphology
Q Science > QL Zoology > QL0801 Anatomy
|Depositing User:||Grecia GarciaGarcia|
|Date Deposited:||19 Aug 2011 10:43|
|Last Modified:||30 Nov 2012 16:55|
|Google Scholar:||8 Citations|