Severity of systemic inflammatory response syndrome (SIRS) affects the blood levels of circulating inflammatory-relevant miRNAs

Caserta, Stefano, Mengozzi, Manuela, Kern, Florian, Newbury, Sarah F, Ghezzi, Pietro and Llewelyn, Martin J (2018) Severity of systemic inflammatory response syndrome (SIRS) affects the blood levels of circulating inflammatory-relevant miRNAs. Frontiers in Immunology, 9 (1977). ISSN 1664-3224

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Abstract

The systemic inflammatory response syndrome (SIRS) is a potentially lethal response triggered by diverse forms of tissue injury and infection. When systemic inflammation is triggered by infection the term sepsis is used. Understanding how inflammation is mediated and regulated is of enormous medical importance. We previously demonstrated that circulating inflammatory-relevant micro-RNAs (CIR-miRNAs) are candidate biomarkers for differentiating sepsis from SIRS. Here we set out to determine how CIR-miRNA levels reflect SIRS severity and whether they derive from activated immune cells. Clinical disease severity scores and markers of red blood cell (RBC) damage or immune cell activation were correlated with CIR-miRNA levels in patients with SIRS and sepsis. The release of CIR-miRNAs modulated during SIRS was assessed in immune cell cultures. We show that severity of non-infective SIRS, but not sepsis is reflected in the levels of miR-378a-3p, miR-30a-5p, miR-30d-5p, and miR-192-5p. These CIR-miRNA levels positively correlate with levels of the redox biomarker, peroxiredoxin-1 (Prdx-1), which has previously been shown to be released by immune cells during inflammation. Furthermore, in vitro activated immune cells produce SIRS-associated miR-378a-3p, miR-30a-5p, miR-30d-5p, and miR-192-5p. Our study furthers the understanding of the origin, role and trafficking of CIR-miRNAs as potential regulators of inflammation.

Item Type: Article
Schools and Departments: Brighton and Sussex Medical School > Clinical and Experimental Medicine
Brighton and Sussex Medical School > Global Health and Infection
Subjects: R Medicine
R Medicine > RB Pathology
Related URLs:
Depositing User: Pietro Ghezzi
Date Deposited: 21 Dec 2017 10:51
Last Modified: 27 Feb 2018 13:51
URI: http://sro.sussex.ac.uk/id/eprint/72413

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