Two-photon fluorescence imaging with 30 fs laser system tunable around 1 micron

Resan, Bojan, Aviles-Espinosa, Rodrigo, Kurmulis, Sarah, Licea-Rodriguez, Jacob, Brunner, Felix, Rohrbacher, Andreas, Artigas, David, Loza-Alvarez, Pablo and Weingarten, Kurt J (2014) Two-photon fluorescence imaging with 30 fs laser system tunable around 1 micron. Optics Express, 22 (13). pp. 16456-16461. ISSN 1094-4087

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Abstract

We developed a low-cost, low-noise, tunable, high-peak-power, ultrafast laser system based on a SESAM-modelocked, solid-state Yb tungstate laser plus spectral broadening via a microstructured fiber followed by pulse compression. The spectral selection, tuning, and pulse compression are performed with a simple prism compressor. The output pulses are tunable from 800 to 1250 nm, with the pulse duration down to 25 fs, and average output power up to 150 mW, at 80 MHz pulse repetition rate. We introduce the figure of merit (FOM) for the two-photon and multi-photon imaging (or other nonlinear processes), which is a useful guideline in discussions and for designing the lasers for an improved microscopy signal. Using a 40 MHz pulse repetition rate laser system, with twice lower FOM, we obtained high signal-to-noise ratio two-photon fluorescence images with or without averaging, of mouse intestine section and zebra fish embryo. The obtained images demonstrate that the developed system is capable of nonlinear (TPE, SHG) imaging in a multimodal operation. The system could be potentially used in a variety of other techniques including, THG, CARS and applications such as nanosurgery.

Item Type: Article
Keywords: Ultrafast technology; Ultrafast lasers; Nonlinear microscopy
Schools and Departments: School of Engineering and Informatics > Engineering and Design
Research Centres and Groups: Sensor Technology Research Centre
Subjects: Q Science > QC Physics > QC0350 Optics. Light
Q Science > QC Physics > QC0350 Optics. Light > QC0450 Spectroscopy
T Technology > TA Engineering (General). Civil engineering (General) > TA0164 Bioengineering
T Technology > TA Engineering (General). Civil engineering (General) > TA1501 Applied optics. Photonics
Depositing User: Rodrigo Amador Aviles-Espinosa
Date Deposited: 19 Dec 2017 14:59
Last Modified: 19 Dec 2017 14:59
URI: http://sro.sussex.ac.uk/id/eprint/72329

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