[Protocol] Colony polymerase chain reaction with Schizosaccharomyces pombe

Murray, Johanne M, Watson, Adam T and Carr, Antony M (2016) [Protocol] Colony polymerase chain reaction with Schizosaccharomyces pombe. Cold Spring Harbor Protocols, 2016 (5). ISSN 1559-6095

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Abstract

When screening a large number of individual Schizosaccharomyces pombe strains by polymerase chain reaction (PCR), a rapid “colony PCR” approach may be used. Numerous colony PCR protocols are available, and fundamental to them all is that the colony must be fresh (grown overnight) and that as few cells as possible are used. In this protocol, we present three reliable methods for preparing S. pombe cells for colony PCR.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Research Centres and Groups: Genome Damage and Stability Centre
Subjects: Q Science > Q Science (General)
Depositing User: Sarah Frances
Date Deposited: 13 Feb 2018 12:29
Last Modified: 16 Apr 2018 15:25
URI: http://sro.sussex.ac.uk/id/eprint/72034

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