Murine norovirus 1 (MNV1) replication induces translational control of the host by regulating eIF4E activity during infection

Royall, Elizabeth, Doyle, Nicole, Abdul-Wahab, Azimah, Emmott, Ed, Morley, Simon J, Goodfellow, Ian, Roberts, Lisa O and Locker, Nicolas (2015) Murine norovirus 1 (MNV1) replication induces translational control of the host by regulating eIF4E activity during infection. Journal of Biological Chemistry, 290 (8). pp. 4748-58. ISSN 0021-9258

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Abstract

Protein synthesis is a tightly controlled process responding to several stimuli, including viral infection. As obligate intracellular parasites, viruses depend on the translation machinery of the host and can manipulate it by affecting the availability and function of specific eukaryotic initiation factors (eIFs). Human norovirus is a member of the Caliciviridae family and is responsible for gastroenteritis outbreaks. Previous studies on feline calicivirus and murine norovirus 1 (MNV1) demonstrated that the viral protein, genome-linked (VPg), acts to direct translation by hijacking the host protein synthesis machinery. Here we report that MNV1 infection modulates the MAPK pathway to activate eIF4E phosphorylation. Our results show that the activation of p38 and Mnk during MNV1 infection is important for MNV1 replication. Furthermore, phosphorylated eIF4E relocates to the polysomes, and this contributes to changes in the translational state of specific host mRNAs. We propose that global translational control of the host by eIF4E phosphorylation is a key component of the host-pathogen interaction.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Research Centres and Groups: Gene Expression Research Group
Depositing User: Simon Morley
Date Deposited: 11 Dec 2017 16:57
Last Modified: 11 Dec 2017 16:57
URI: http://sro.sussex.ac.uk/id/eprint/71984
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