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Murine norovirus 1 (MNV1) replication induces translational control of the host by regulating eIF4E activity during infection
journal contribution
posted on 2023-06-09, 09:19 authored by Elizabeth Royall, Nicole Doyle, Azimah Abdul-Wahab, Ed Emmott, Simon Morley, Ian Goodfellow, Lisa O Roberts, Nicolas LockerProtein synthesis is a tightly controlled process responding to several stimuli, including viral infection. As obligate intracellular parasites, viruses depend on the translation machinery of the host and can manipulate it by affecting the availability and function of specific eukaryotic initiation factors (eIFs). Human norovirus is a member of the Caliciviridae family and is responsible for gastroenteritis outbreaks. Previous studies on feline calicivirus and murine norovirus 1 (MNV1) demonstrated that the viral protein, genome-linked (VPg), acts to direct translation by hijacking the host protein synthesis machinery. Here we report that MNV1 infection modulates the MAPK pathway to activate eIF4E phosphorylation. Our results show that the activation of p38 and Mnk during MNV1 infection is important for MNV1 replication. Furthermore, phosphorylated eIF4E relocates to the polysomes, and this contributes to changes in the translational state of specific host mRNAs. We propose that global translational control of the host by eIF4E phosphorylation is a key component of the host-pathogen interaction.
History
Publication status
- Published
Journal
Journal of Biological ChemistryISSN
0021-9258Publisher
American Society for Biochemistry and Molecular BiologyExternal DOI
Issue
8Volume
290Page range
4748-58Department affiliated with
- Biochemistry Publications
Research groups affiliated with
- Gene Expression Research Group Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2017-12-11Usage metrics
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