Synthesis and cellular characterization of various nano-assemblies of cell penetrating peptide-epirubicin-polyglutamate conjugates for the enhancement of antitumor activity

Mohammadi, Samaneh, Zakeri-Milani, Parvin, Golkar, Nasim, Farkhani, Samad Mussa, Shirani, Ali, Shahbazi Mojarrad, Javid, Nokhodchi, Ali and Valizadeh, Hadi (2017) Synthesis and cellular characterization of various nano-assemblies of cell penetrating peptide-epirubicin-polyglutamate conjugates for the enhancement of antitumor activity. Artificial Cells, Nanomedicine, and Biotechnology. pp. 1-14. ISSN 2169-1401

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Abstract

A new class of cell penetrating peptides (CPPs) named peptide amphiphile was designed to improve the intracellular uptake and antitumor activity of epirubicin (EPR). Various amphiphilic CPPs were synthesized by solid phase peptide synthesis method and were chemically conjugated to EPR. Their corresponding nanoparticles (CPPs-E4 and CPPs-E8) were prepared via non-covalent binding of the peptides and polyanions. Cytotoxicity and anti-proliferative activity were evaluated by MTT assay. Cellular uptake was examined by flow cytometry and fluorescence microscopy. The CPPs exhibited slight cytotoxicity. Binding of polyglutamate to CPPs (CPPs-E4 and CPPs-E8 nanoparticles) decreased their cytotoxicity. CPPs-E8 nanoparticles showed lower cytotoxicity than CPPs-E4 nanoparticles. Cellular uptake of K3W4K3-E8, K2W4K2-E8 and W3K4W3-E8 reached 100% with no difference between each of the mentioned CPPs and its nanoparticle at 50 µM. The anti-proliferative activity of EPR was enhanced following conjugation to peptides and nanoparticles at 25 µM. CPPs-EPR-E4 and –E8 nanoparticles displayed higher anti-proliferative activity than CPPs-EPR at 25 µM. CPPs–E8-EPR nanoparticles showed higher anti-proliferative activity than CPPs–E4-EPR. K3W4K3-E8-EPR nanoparticles exhibited the highest anti-proliferative activity at 25 µM. The synthesized peptide nanoparticles are proposed as suitable carriers for improving the intracellular delivery of EPR into tumor cells with low cytotoxicity and high antitumor activity.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
Research Centres and Groups: Analysis and Partial Differential Equations Research Group
Subjects: R Medicine > RS Pharmacy and materia medica > RS0153 Materia medica > RS0192 Pharmaceutical technology
Depositing User: Ali Nokhodchi
Date Deposited: 18 Dec 2017 15:15
Last Modified: 18 Dec 2017 15:15
URI: http://sro.sussex.ac.uk/id/eprint/71899

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