[Protocol]: Transformation of schizosaccharomyces pombe: protoplast procedure

Murray, Johanne M, Watson, Adam T and Carr, Antony M (2016) [Protocol]: Transformation of schizosaccharomyces pombe: protoplast procedure. Cold Spring Harbour Protocols, 2016 (4). ISSN 1559-6095

[img] PDF - Accepted Version
Restricted to SRO admin only

Download (196kB)

Abstract

Transformation of Schizosaccharomyces pombe with DNA requires the conditioning of cells to promote DNA uptake followed by cell growth under conditions that select and maintain the plasmid or integration event. The three main methodologies are electroporation, treatment with lithium cations, and transformation of protoplasts. The protocol for protoplast transformation, which is described here, is more complicated than those for electroporation or lithium acetate and thus less often used. However, for some strains, it remains the only reliable protocol.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Research Centres and Groups: Genome Damage and Stability Centre
Depositing User: Paula Amiet-West
Date Deposited: 12 Dec 2017 12:27
Last Modified: 12 Dec 2017 12:27
URI: http://sro.sussex.ac.uk/id/eprint/71866

View download statistics for this item

📧 Request an update
Project NameSussex Project NumberFunderFunder Ref
Replication stalling in a palindromeG0181ASSOCIATION FOR INTERNATIONAL CANCER RESEARCH10-0273
Smc5/6 and replication fork stabilityG0179MRC-MEDICAL RESEARCH COUNCILG0901011