[Protocol]Transformation of schizosaccharomyces pombe: lithium acetate/ dimethyl sulfoxide procedure

Murray, Johanne M, Watson, Adam T and Carr, Antony M (2016) [Protocol]Transformation of schizosaccharomyces pombe: lithium acetate/ dimethyl sulfoxide procedure. Cold Spring Harbour Protocols, 2016 (4). ISSN 1559-6095

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Abstract

Transformation of Schizosaccharomyces pombe with DNA requires the conditioning of cells to promote DNA uptake followed by cell growth under conditions that select and maintain the plasmid or integration event. The three main methodologies are electroporation, treatment with lithium cations, and transformation of protoplasts. The lithium acetate method described here is widely used because it is simple and reliable.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Research Centres and Groups: Genome Damage and Stability Centre
Depositing User: Paula Amiet-West
Date Deposited: 12 Dec 2017 12:23
Last Modified: 13 Dec 2017 15:18
URI: http://sro.sussex.ac.uk/id/eprint/71862

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