[Protocol]: Transformation of schizosaccharomyces pombe: electroporation procedure

Murray, Johanne, Watson, Adam and Carr, Antony (2016) [Protocol]: Transformation of schizosaccharomyces pombe: electroporation procedure. Cold Spring Harbor Protocols, 2016 (4). ISSN 1559-6095

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Abstract

Transformation of Schizosaccharomyces pombe with DNA requires the conditioning of cells to promote DNA uptake followed by cell growth under conditions that select and maintain the plasmid or integration event. The three main methodologies are electroporation, treatment with lithium cations, and transformation of protoplasts. This protocol describes transformation by electroporation. It involves pretreating S. pombe cells with dithiothreitol (DTT), which increases the transformation efficiency once the electric pulse is applied.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Research Centres and Groups: Genome Damage and Stability Centre
Subjects: Q Science
Q Science > Q Science (General)
Depositing User: Paula Amiet-West
Date Deposited: 12 Dec 2017 13:50
Last Modified: 26 Mar 2018 13:47
URI: http://sro.sussex.ac.uk/id/eprint/71803

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