An mRNA decapping mutant deficient in P body assembly limits mRNA stabilization in response to osmotic stress

Huch, Susanne and Nissan, Tracy (2017) An mRNA decapping mutant deficient in P body assembly limits mRNA stabilization in response to osmotic stress. Scientific reports, 7. p. 44395. ISSN 2045-2322

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Abstract

Yeast is exposed to changing environmental conditions and must adapt its genetic program to provide a homeostatic intracellular environment. An important stress for yeast in the wild is high osmolarity. A key response to this stress is increased mRNA stability primarily by the inhibition of deadenylation. We previously demonstrated that mutations in decapping activators (edc3∆ lsm4∆C), which result in defects in P body assembly, can destabilize mRNA under unstressed conditions. We wished to examine whether mRNA would be destabilized in the edc3∆ lsm4∆C mutant as compared to the wild-type in response to osmotic stress, when P bodies are intense and numerous. Our results show that the edc3∆ lsm4∆C mutant limits the mRNA stability in response to osmotic stress, while the magnitude of stabilization was similar as compared to the wild-type. The reduced mRNA stability in the edc3∆ lsm4∆C mutant was correlated with a shorter PGK1 poly(A) tail. Similarly, the MFA2 mRNA was more rapidly deadenylated as well as significantly stabilized in the ccr4∆ deadenylation mutant in the edc3∆ lsm4∆C background. These results suggest a role for these decapping factors in stabilizing mRNA and may implicate P bodies as sites of reduced mRNA degradation.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: Tracy Nissan
Date Deposited: 17 Nov 2017 15:48
Last Modified: 17 Nov 2017 15:48
URI: http://sro.sussex.ac.uk/id/eprint/71341

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